Work Packages

The networking strategy will be promoted by establishing a number of work packages (WP). Beside the WP for management, training and dissemination (WP 1, 6 and 7), there are 4 research WP with synergistically overlapping research activities:

WORK PACKAGE 2- Anti-oxidant and immune-modulatory supplements for animal health

The experimental work of WP 2 involves ESR 1, ESR3, ESR4 and ESR7, who will investigate the impact of dietary supplementation with polyphenols and other anti-oxidants and immunomodulatory molecules in pigs and chicken.

The WP is divided in three tasks:

Task 2.1 Effects of the n-6/n-3 fatty acids ratio in the diet of pregnant sows on piglet microbiome and immunity (involving ESR1).

Task 2.2 Proteome and metabolome responses and nutritional biomarkers following dietary supplementation with antioxidants and anti-inflammatories in chicken (involving ESR3, ESR4).

Task 2.3 Bacterial, fungal and archaeal components of the gut microbiota and their impact on animal nutrition (involving ESR7).

Objectives of WP2 are:

To test at the molecular level the impact of dietary treatments (antioxidant supplements or different dietary n-3 : n-6 fatty acids ratios and immunomodulatory molecules) in chicken and pigs on growth performances

To describe, at cellular and molecular levels, the change of gastro-intestinal mucosa and other tissues caused by the dietary treatments

To describe the impact of the treatments on the microbiome and the mycobiome

WORK PACKAGE 3 - In vitro models of animal nutrition

The experimental work of WP3 involves ESR 2 and ESR9, who will use in vitro models of intestinal and white blood cells from the 3 animal species involved for investigating the impact of the compounds tested in some of the in vivo studies in WP 2 or in WP 4 (e.g. plant antioxidants, specific fatty acids). In addition, milk exosome preparations, as provided from ESR6 as part of WP2, will also be utilized to characterize exosomes from sow milk to refine the role of miRNA on exosome composition and milk quality.

The WP is divided in three tasks:

Task 3.1 Nutrition and immunity: Molecular approaches (involving ESR2).

Task 3.2 Development of in vitro models for intestinal function in animal nutrition (involving ESR9).

Task 3.3 miRNAomics of sow’s milk exosomes (involving ESR6).

Objectives of WP3 are:

To develop in vitro models to assess the impact of molecules used during MANNA on innate immunity.

To characterize the structure of milk exosomes from sow and cows

WORK PACKAGE 4 - Improving the knowledge about the activity spectra of "classical" supplements for animal health and productivity

The experimental work of WP 4 involves ESR 5, ESR 8 and ESR 10, who will investigate the effects of dietary supplementation with the amino acid Methionine (Met), essential fatty acids (EFA), polyunsaturated fatty acids (PUFA), and of conjugated linoleic acids (CLA) in dairy cattle and in pigs, respectively.

The WP is divided into three tasks:

Task 4.1 Testing the effect of increased Met intakes on milk production and composition, on metabolism, health and fertility in dairy cows (involving ESR5).

Task 4.2 Evaluating the need for and the benefit of EFA and of CLA in dairy cows and their calves (involving ESR 10)

Task 4.3 Testing the molecular effects of dietary fat rich in PUFA (mainly n-6 linoleic acid) on growth performance and carcass quality in pigs (involving ESR8)

Objectives of WP4 are

To evaluate the effects of increased Met intakes on metabolism and fertility in dairy cows during the first 20 weeks of lactation

To address the requirement for EFA and CLA (alone and in combination) in dairy cows receiving a diet low in EFA and also in their calves

To determine the effects of specific dietary fatty acids/lipids on growth performance and meat and fat quality in pigs

WORK PACKAGE 5 - Development of an immuno-metabolic bioinformatics platform

The experimental work of WP 5 involves ESR 11, who will develop algorithms for data analysis by using cognitive computing.

The WP is divided into three tasks:

Task 5.1 Understanding the analytic need and map the resources of data gathering (involving ESR11 with contributions from all ESRs)

Task 5.2 repository building for Omics data and immuno-metabolic informatics (involving ESR11 with contributions from all ESRs)

Task 5.3 Development of an integrated software platform – OMnalysis (involving ESR11 with contributions from all ESRs)

Objectives of WP5 are

To integrate omics data generated from MANNA

To build a data repository and text mining tool for omics analyses

To create an integrated software platform OMnalysis for Omics data analysis to extract biological relevance of putative biomarkers

This project has received funding from the European Union’s Horizon 2020 research and innovation programme H2020-MSCA- ITN-2017- EJD: Marie Skłodowska-Curie Innovative Training Networks (European Joint Doctorate) – Grant agreement nº: 765423